tRNA curation question

[Harold Drabkin]

1. The tRNA does not have the "aminoacyl-tRNA ligase activity ". What 
amino acid it accepts will depend on what sequence the tRNA has, 
especially in it's anticodon, to see which codon it would decode 
(although even THAT is not 100% in some genomes).

I think at the moment, there is really only one function term that the 
tRNA has: GO:0030533.

2. Process
A tRNA is not involved in tRNA aminoacylation. It is the substrate of 
the process. . The aminoacyl-tRNA synthetase is involved in that process.
However, it is involved in translation (*GO:0043037*); most likely 
elongation, unless it's an initiator methionine tRNA, in which case it 
would be involved in elongation;

3. component: cytoplasm or mitochondrion or chloroplast. The latter two 
DO encode some of their own tRNA. But telling by the sequence may be 
difficult. They are NOT a component of the ribosome or peptidyl 
transferase.


Harold

Pankaj Jaiswal wrote:
>
> Hi,
>
> This is the first time I am trying to do a tRNA curation and I need 
> help on the possible GO terms. As I understand my choices of terms are 
> as follows.
>
> -Pankaj
>
> ------------------
>
> e.g. I have a gene product tRNA-Cysteine
>
> Function: GO:0030533 : triplet codon-amino acid adaptor activity
>     GO:0004817 : cysteine-tRNA ligase activity
>     GO:0004812 : aminoacyl-tRNA ligase activity
>     GO:0003729 : mRNA binding
> Process: GO:0006423 : cysteinyl-tRNA aminoacylation
>     GO:0006418 : tRNA aminoacylation for protein translation
> Component: ??
>     ribosome
>     peptidyl transferase
>     
>
>