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tRNA curation question
Tanya Berardini
tberardi at acoma.Stanford.EDU
Tue Mar 14 11:20:01 PST 2006
We've used 'translational elongation' GO:0006414 for the process annotation.
Tanya
Harold Drabkin wrote:
> 1. The tRNA does not have the "aminoacyl-tRNA ligase activity ". What
> amino acid it accepts will depend on what sequence the tRNA has,
> especially in it's anticodon, to see which codon it would decode
> (although even THAT is not 100% in some genomes).
>
> I think at the moment, there is really only one function term that the
> tRNA has: GO:0030533.
>
> 2. Process
> A tRNA is not involved in tRNA aminoacylation. It is the substrate of
> the process. . The aminoacyl-tRNA synthetase is involved in that process.
> However, it is involved in translation (*GO:0043037*); most likely
> elongation, unless it's an initiator methionine tRNA, in which case it
> would be involved in elongation;
>
> 3. component: cytoplasm or mitochondrion or chloroplast. The latter two
> DO encode some of their own tRNA. But telling by the sequence may be
> difficult. They are NOT a component of the ribosome or peptidyl
> transferase.
>
>
> Harold
>
> Pankaj Jaiswal wrote:
>>
>> Hi,
>>
>> This is the first time I am trying to do a tRNA curation and I need
>> help on the possible GO terms. As I understand my choices of terms are
>> as follows.
>>
>> -Pankaj
>>
>> ------------------
>>
>> e.g. I have a gene product tRNA-Cysteine
>>
>> Function: GO:0030533 : triplet codon-amino acid adaptor activity
>> GO:0004817 : cysteine-tRNA ligase activity
>> GO:0004812 : aminoacyl-tRNA ligase activity
>> GO:0003729 : mRNA binding
>> Process: GO:0006423 : cysteinyl-tRNA aminoacylation
>> GO:0006418 : tRNA aminoacylation for protein translation
>> Component: ??
>> ribosome
>> peptidyl transferase
>>
>>
--
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Tanya Berardini, Ph.D. tberardi at acoma.stanford.edu
The Arabidopsis Information Resource FAX: (650) 325-6857
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